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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/11/2020 |
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Data da última atualização: |
23/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GRUNENNVALDT, R. L.; DEGENHARDT, J.; BROOKS, P.; TOMASI, J. de C.; HANSEL, F. A.; TRAN, T.; GOMES, E. N.; DESCHAMPS, C. |
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Afiliação: |
RENATA LÚCIA GRUNENNVALDT, UFPR; JULIANA DEGENHARDT, CNPF; PETER BROOKS, University of The Sunshine Coast; JÉSSICA DE CÁSSIA TOMASI, UFPR; FABRICIO AUGUSTO HANSEL, CNPF; TRONG TRAN, University of The Sunshine Coast; ERIK N. GOMES, The State University of New Jersey; CÍCERO DESCHAMPS, UFPR. |
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Título: |
Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p. |
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DOI: |
https://doi.org/10.1590/0001-3765202020181251 |
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Idioma: |
Inglês |
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Conteúdo: |
Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine. |
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Palavras-Chave: |
Acúmulo de biomassa; Atividade antioxidante; Biomass accumulation; Erva mate; Plant growth regulators. |
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Thesagro: |
Ilex Paraguariensis; Regulador de Crescimento; Sacarose. |
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Thesaurus Nal: |
Antioxidant activity; Sucrose; Yerba mate. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217736/1/Juliana-Grunennvaldt-et-al-2020.pdf
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Marc: |
LEADER 02511naa a2200349 a 4500
001 2126527
005 2020-11-23
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/0001-3765202020181251$2DOI
100 1 $aGRUNENNVALDT, R. L.
245 $aCallus culture as a new approach for the production of high added value compounds in Ilex paraguariensis$bgenotype infl uence, medium optimization and compounds identifi cation.$h[electronic resource]
260 $c2020
520 $aIlex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine.
650 $aAntioxidant activity
650 $aSucrose
650 $aYerba mate
650 $aIlex Paraguariensis
650 $aRegulador de Crescimento
650 $aSacarose
653 $aAcúmulo de biomassa
653 $aAtividade antioxidante
653 $aBiomass accumulation
653 $aErva mate
653 $aPlant growth regulators
700 1 $aDEGENHARDT, J.
700 1 $aBROOKS, P.
700 1 $aTOMASI, J. de C.
700 1 $aHANSEL, F. A.
700 1 $aTRAN, T.
700 1 $aGOMES, E. N.
700 1 $aDESCHAMPS, C.
773 $tAnais da Academia Brasileira de Ciências$gv. 92, n. 3, e20181251, 2020. 16 p.
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
24/09/2009 |
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Data da última atualização: |
24/09/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
PEREIRA, A. I. A.; MALAQUIAS, J. B.; RAMALHO, F. de S.; ZANUNCIO, J. C. |
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Afiliação: |
Alexandre Igor Azevedo Pereira, UFV; José Bruno Malaquias; Francisco de Sousa Ramalho, Embrapa Algodão; José Cola Zanuncio, UFV. |
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Título: |
Variação no número de ovos por fêmea do predador podisus nigrispinus (heteroptera: pentatomidae) mantido sob diferentes fotoperíodos: um estudo comparativo. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. |
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ISSN: |
2175-2311 |
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Idioma: |
Português |
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Palavras-Chave: |
Asopinae. |
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Thesagro: |
Controle Biológico; Entomologia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00767naa a2200193 a 4500
001 1428555
005 2009-09-24
008 2009 bl uuuu u00u1 u #d
022 $a2175-2311
100 1 $aPEREIRA, A. I. A.
245 $aVariação no número de ovos por fêmea do predador podisus nigrispinus (heteroptera$bpentatomidae) mantido sob diferentes fotoperíodos: um estudo comparativo.
260 $c2009
650 $aControle Biológico
650 $aEntomologia
653 $aAsopinae
700 1 $aMALAQUIAS, J. B.
700 1 $aRAMALHO, F. de S.
700 1 $aZANUNCIO, J. C.
773 $tIn: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009.
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